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Nat Commun. 2018 Nov 12;9(1):4762. doi: 10.1038/s41467-018-07246-2.

Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

Author information

1
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
2
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. sjakobs@gwdg.de.
3
Department of Neurology, University Medical Center Göttingen, Göttingen, Germany. sjakobs@gwdg.de.

Abstract

The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

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