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Transl Oncol. 2019 Feb;12(2):226-235. doi: 10.1016/j.tranon.2018.10.006. Epub 2018 Nov 10.

Multi-Spectral Fluorescence Imaging of Colon Dysplasia InVivo Using a Multi-Spectral Endoscopy System.

Author information

1
Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea; Department of Medicine, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
2
Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
3
School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea.
4
Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju, Institute of Science and Technology, Gwangju 61005, South Korea.
5
Discovery and Analytic Solution, PerkinElmer Korea, Seoul 08380, South Korea.
6
Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, South Korea.
7
Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
8
School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea; Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju, Institute of Science and Technology, Gwangju 61005, South Korea.
9
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
10
Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
11
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea; Department of Gastroenterology and Convergence Medicine, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
12
Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea; Department of Gastroenterology and Convergence Medicine, University of Ulsan College of Medicine, Seoul 138-736, South Korea. Electronic address: sykim3yk@amc.seoul.kr.
13
Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, South Korea; Department of Gastroenterology and Convergence Medicine, University of Ulsan College of Medicine, Seoul 138-736, South Korea. Electronic address: sjmyung@amc.seoul.kr.

Abstract

BACKGROUND AND STUDY AIM:

To develop a molecular imaging endoscopic system that eliminates tissue autofluorescence and distinguishes multiple fluorescent markers specifically on the cancerous lesions.

METHODS:

Newly developed multi-spectral fluorescence endoscope device has the potential to eliminate signal interference due to autofluorescence and multiplex fluorophores in fluorescent probes. The multiplexing capability of the multi-spectral endoscope device was demonstrated in the phantom studies and multi-spectral imaging with endoscopy and macroscopy was performed to analyze fluorescence signals after administration of fluorescent probe that targets cancer in the colon. Because of the limitations in the clinical application using rigid-type small animal endoscope, we developed a flexible channel insert-type fluorescence endoscope, which was validated on the colonoscopy of dummy and porcine model.

RESULTS:

We measured multiple fluorescent signals simultaneously, and the fluorescence spectra were unmixed to separate the fluorescent signals of each probe, in which multiple fluorescent probes clearly revealed spectral deconvolution at the specific targeting area in the mouse colon. The positive area of fluorescence signal for each probe over the whole polyp was segmented with analyzing software, and showed distinctive patterns and significantly distinguishable values: 0.46 ± 0.04, 0.39 ± 0.08 and 0.73 ± 0.12 for HMRG, CET-553 and TRA-675 probes, respectively. The spectral unmixing was finally demonstrated in the dummy and porcine model, corroborating the targeted multi-spectral fluorescence imaging of colon dysplasia.

CONCLUSION:

The multi-spectral endoscopy system may allow endoscopists to clearly identify cancerous lesion that has different patterns of various target expression using multiple fluorescent probes.

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