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BMC Complement Altern Med. 2018 Nov 9;18(1):299. doi: 10.1186/s12906-018-2359-6.

Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models.

Thabet AA1, Youssef FS1, Korinek M2,3,4,5, Chang FR2,6, Wu YC2,7,8, Chen BH3,8,9, El-Shazly M10,11, Singab ANB12, Hwang TL13,14,15,16,17.

Author information

1
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, Cairo, 11566, Egypt.
2
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
3
Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
4
Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
5
Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, 33302, Taiwan.
6
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, 11221, Taiwan.
7
Research Center for Natural Products & Drug Development, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
8
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, 80708, Taiwan.
9
The Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
10
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, Cairo, 11566, Egypt. mohamed.elshazly@pharma.asu.edu.eg.
11
Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, 11835, Egypt. mohamed.elshazly@pharma.asu.edu.eg.
12
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbassia, Cairo, 11566, Egypt. dean@pharma.asu.edu.eg.
13
Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan. htl@mail.cgu.edu.tw.
14
Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, 33302, Taiwan. htl@mail.cgu.edu.tw.
15
Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, 33305, Taiwan. htl@mail.cgu.edu.tw.
16
Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan, 33302, Taiwan. htl@mail.cgu.edu.tw.
17
Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. htl@mail.cgu.edu.tw.

Abstract

BACKGROUND:

Brachychiton rupestris and Brachychiton discolor (Malvaceae) are ornamental trees native to Australia. Some members of Brachychiton and its highly related genus, Sterculia, are employed in traditional medicine for itching, dermatitis and other skin diseases. However, scientific studies on these two genera are scarce. Aiming to reveal the scientific basis of the folk medicinal use of these plants, the cytotoxicity, anti-inflammatory and anti-allergic activities of Brachychiton rupestris and Brachychiton discolor leaves extracts and fractions were evaluated. Also, phytochemical investigation of B. rupestris was performed to identify the compounds exerting the biological effect.

METHODS:

Extracts as well as fractions of Brachychiton rupestris and Brachychiton discolor were tested for their cytotoxicity versus hepatoma HepG2, lung A549, and breast MDA-MB-231 cancer cell lines. Assessment of the anti-allergic activity was done using degranulation assay in RBL-2H3 mast cells. Anti-inflammatory effect was tested by measuring the suppression of superoxide anion production as well as elastase release in fMLF/CB-induced human neutrophils. Phytochemical investigation of the n-hexane, dichloromethane and ethyl acetate fractions of B. rupestris was done using different chromatographic and spectroscopic techniques.

RESULTS:

The tested samples showed no cytotoxicity towards the tested cell lines. The nonpolar fractions of both B. rupestris and B. discolor showed potent anti-allergic potency by inhibiting the release of β-hexosaminidase. The dichloromethane fraction of both species exhibited the highest anti-inflammatory activity by suppressing superoxide anion generation and elastase release with IC50 values of 2.99 and 1.98 μg/mL, respectively for B. rupestris, and 0.78 and 1.57 μg/mL, respectively for B. discolor. Phytochemical investigation of various fractions of B. rupestris resulted in the isolation of β-amyrin acetate (1), β-sitosterol (2) and stigmasterol (3) from the n-hexane fraction. Scopoletin (4) and β-sitosterol-3-O-β-D-glucoside (5) were obtained from the dichloromethane fraction. Dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside (6) and dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (7) were separated from the ethyl acetate fraction. Scopoletin (4) showed anti-allergic and anti-inflammatory activity.

CONCLUSIONS:

It was concluded that the nonpolar fractions of both Brachychiton species exhibited anti-allergic and anti-inflammatory activities.

KEYWORDS:

Anti-allergic; Anti-inflammatory; Brachychiton discolor; Brachychiton rupestris; Cytotoxicity; Phytochemistry

PMID:
30413192
PMCID:
PMC6230296
DOI:
10.1186/s12906-018-2359-6
[Indexed for MEDLINE]
Free PMC Article

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