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Bioanalysis. 2018 Nov 6. doi: 10.4155/bio-2018-0184. [Epub ahead of print]

A UHPLC-MS/MS method for the quantification of Δ9-tetrahydrocannabinol and cannabidiol in decoctions and in plasma samples for therapeutic monitoring of medical cannabis.

Author information

1
Department of Services and Diagnostic Laboratories, Central Laboratory of Analyses Unit, IRCCS Istituto Giannina Gaslini, 16143 Genoa, Italy.
2
Department of Internal Medicine, Pharmacology & Toxicology Unit, University of Genoa, 16100 Genoa, Italy.
3
Paediatric Chronic Pain & Palliative Care Service, IRCCS Istituto Giannina Gaslini, 16143 Genoa, Italy.
4
Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics & Maternal & Children's Sciences, Child Neuropsychiatry Unit, IRCCS Giannina Gaslini Institute, 16143 Genoa, Italy.

Abstract

AIM:

Monitoring of blood levels of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) is necessary for optimization of administration of medical cannabis. We describe the validation of a ultra-HPLC-MS/MS method for quantifying THC and CBD from plasma and decoctions and its application for therapeutic drug monitoring.Materials & methods: Analyses were performed by using a TSQ Quantiva™ Triple Quadrupole coupled to a Ultimate 3000 UHPLC system with atmospheric pressure chemical ionization after sample preparation with a straightforward method with deuterated internal standards.

RESULTS:

The method has been validated following EMA guidelines and is linear in plasma from 0.16 to 10 ng/ml for both THC and CBD and in decoctions from 4.7 to 600 ng/ml.

CONCLUSION:

Given the unpredictable pharmacokinetic behavior of THC and CBD in patients, monitoring of plasma concentrations is strongly recommended for patients under treatment with medical cannabis.

KEYWORDS:

CBD; LC–MS/MS; THC; cannabis; chromatography-tandem mass spectrometry; therapeutic drug monitoring

PMID:
30412688
DOI:
10.4155/bio-2018-0184

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