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J Agric Food Chem. 2018 Nov 21. doi: 10.1021/acs.jafc.8b05272. [Epub ahead of print]

Blueberry Polyphenols Ameliorate Visible Light and Lipid-Induced Injury of Retinal Pigment Epithelial Cells.

Liu Y1,2,3, Liu M4, Chen Q1, Liu GM1,2,3, Cao MJ1,2,3, Sun L1,2,3, Lu Z1, Guo C1.

Author information

1
College of Food and Biological Engineering , Jimei University , Xiamen 361021 , Fujian People's Republic of China.
2
Xiamen Key Laboratory of Marine Functional Food , Jimei University , Xiamen 361021 , Fujian People's Republic of China.
3
Fujian Provincial Engineering Technology Research Center of Marine Functional Food , Jimei University , Xiamen 361021 , Fujian People's Republic of China.
4
Academy of State Administration of Grain , Beijing 100037 , People's Republic of China.

Abstract

Although dietary polyphenols are known to be beneficial to vision, the protective distinctions among different types of polyphenols are unclear. In this work, the visual benefits of various blueberry polyphenols were evaluated using an in vitro model of visible light-lipid-induced injury of retinal pigment epithelial cells. Results showed that, at 10.0 μg/mL, the phenolic acid-rich fraction was superior in inhibiting cell death (93.6% ± 2.8% of cell viability). Anthocyanin- and flavonoid-rich fractions shared similar advantages in preventing the expression of senescence-associated β-galactosidase (34.8% ± 11.1% and 32.2% ± 9.7% of aged cells, respectively) and overexpression of vascular endothelial growth factor (51.8 ± 3.5 and 54.1 ± 6.5 pg/mL, respectively). The flavonoid-rich fraction also showed high activity in ameliorating phagocytosis (70.3% ± 12.6%) and cellular oxidative stress. These results were further confirmed by using the corresponding polyphenol standards. Improved inhibitory effects of polyphenol mixture on cell death and senescence-associated β-galactosidase expression were also observed. Therefore, various polyphenols play diverse roles and exert synergistic effects in nourishing the retina.

KEYWORDS:

anthocyanins; blueberry polyphenols; lipid peroxidation; retinal pigment epithelium cells; visible light

PMID:
30411897
DOI:
10.1021/acs.jafc.8b05272

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