Abstract
A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.
MeSH terms
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Biomarkers, Tumor / blood*
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Biomarkers, Tumor / genetics
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Galactosides / chemistry
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Humans
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Limit of Detection
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MicroRNAs / blood*
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MicroRNAs / genetics
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Nucleic Acid Hybridization / methods*
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Oxazines / chemistry
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Spectrometry, Fluorescence / methods*
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beta-Galactosidase / chemistry
Substances
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Biomarkers, Tumor
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Galactosides
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MIRN451 microRNA, human
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MicroRNAs
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Oxazines
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resorufin galactopyranoside
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beta-Galactosidase