Format

Send to

Choose Destination
Genome Biol. 2018 Nov 8;19(1):192. doi: 10.1186/s13059-018-1530-1.

Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice.

Duan J1,2, Lu G1,2, Hong Y3,2, Hu Q2, Mai X2, Guo J1,2, Si X1,2, Wang F2, Zhang Y4,5,6,7.

Author information

1
Graduate Program, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China.
2
National Institute of Biological Sciences, Beijing, 102206, China.
3
Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, 100871, China.
4
Graduate Program, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China. zhangyu@nibs.ac.cn.
5
Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing, 100871, China. zhangyu@nibs.ac.cn.
6
National Institute of Biological Sciences, Beijing, 102206, China. zhangyu@nibs.ac.cn.
7
Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, 100084, China. zhangyu@nibs.ac.cn.

Abstract

CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules to defined genome regions where it can modulate genetic and epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of CRISPR/dCas9 in animals have been challenged by delivery issues. We generate and characterize a mouse strain with dCas9-EGFP ubiquitously expressed in various tissues. Studying telomere dynamics in these animals reveals surprising results different from those observed in cultured cell lines. The CRISPR/dCas9 knock-in mice provide an important and versatile tool to mechanistically study genome functions in live animals.

KEYWORDS:

CRISPR/dCas9; Genome labeling; Hepatocytes; Knock-in mice; Live imaging; Telomere dynamics; dCas9-EGFP

PMID:
30409154
PMCID:
PMC6225728
DOI:
10.1186/s13059-018-1530-1
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center