Format

Send to

Choose Destination
Elife. 2018 Nov 7;7. pii: e39649. doi: 10.7554/eLife.39649.

CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors.

Author information

1
Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, Lyon, France.
2
Department of Biological Sciences, University of Illinois at Chicago, Chicago, United States.

Abstract

The assembly of neurotransmitter receptors in the endoplasmic reticulum limits the number of receptors delivered to the plasma membrane, ultimately controlling neurotransmitter sensitivity and synaptic transfer function. In a forward genetic screen conducted in the nematode C. elegans, we identified crld-1 as a gene required for the synaptic expression of ionotropic acetylcholine receptors (AChR). We demonstrated that the CRLD-1A isoform is a membrane-associated ER-resident protein disulfide isomerase (PDI). It physically interacts with AChRs and promotes the assembly of AChR subunits in the ER. Mutations of Creld1, the human ortholog of crld-1a, are responsible for developmental cardiac defects. We showed that Creld1 knockdown in mouse muscle cells decreased surface expression of AChRs and that expression of mouse Creld1 in C. elegans rescued crld-1a mutant phenotypes. Altogether these results identify a novel and evolutionarily-conserved maturational enhancer of AChR biogenesis, which controls the abundance of functional receptors at the cell surface.

KEYWORDS:

C. elegans; Cys-loop receptor; acetylcholine receptor (AChR); cell biology; endoplasmic reticulum (ER); mouse; neuromuscular junction (NMJ); neuroscience; protein disulphide isomerase (PDI)

PMID:
30407909
PMCID:
PMC6245729
DOI:
10.7554/eLife.39649
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center