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Lab Chip. 2018 Dec 7;18(23):3539-3549. doi: 10.1039/c8lc00804c. Epub 2018 Nov 8.

Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications.

Author information

1
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn and R&D Center of Healthcare Electronics, Institute of Microelectronics, Chinese Academy of Sciences, Beijing, 100029, China.
2
Department of Neurobiology, Capital Medical University, Beijing, 100069, China and PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China. ch.zhang@pku.edu.cn.
3
School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA.
4
Institute of molecular and medicine, Peking University, Beijing, 100871, China.
5
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn.
6
PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China. ch.zhang@pku.edu.cn.
7
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn and National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing, 100871, China.

Abstract

Parylene-C is a popular polymer material in biomedical applications, with excellent physicochemical properties and microfabrication capability. Like many aromatic polymers, parylene-C also has autofluorescence, which was usually taken as a negative background noise in biomedical detection studies. However, the fluorescence intensity of thin-film (<1 μm) parylene-C was relatively weak, which may be a big limitation in visualization. In this work, we reported a simple annealing method to significantly enhance the fluorescence and achieve sufficient intensity as a visual marker. We studied the behaviors and mechanisms of the enhanced parylene-C fluorescence, then verified the feasibility and reliability of parylene-C for preparing fluorescent pipettes in targeted neuronal electrophysiology, where fluorescent guidance was strongly needed. The powerful parylene-C fabrication technique enables a precisely-controlled conformal coating along with a mass production capability, which further resulted in high-quality electrophysiological recordings of both cultured hippocampal neurons and acute hippocampal brain slices. Moreover, the enhanced parylene-C fluorescence can also be applied in more general biological operations, such as designable fluorescent micro-patterns for visualization in broader biomedical fields.

PMID:
30406244
DOI:
10.1039/c8lc00804c
[Indexed for MEDLINE]

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