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Folia Morphol (Warsz). 2018 Nov 7. doi: 10.5603/FM.a2018.0105. [Epub ahead of print]

Acrylamide adverse cerebellar changes in rats: possible oligodendrogenic role of omega 3 and green tea.

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Departement of Anatomy & Embryology,faculty of Medicine, Cairo University, Kasr Alainy, Cairo, +2 Cairo, Egypt.



Humans are widely exposed to Acrylamide (ACR) and its neurotoxicity is a significant public health issue attracting wide attention.


Investigating ACR-induced adverse cerebellar changes in rats and studying the possible Oligodendrogenic role of Omega 3 and Green Tea.


Twenty four adult albino rats weighing 150 -200gm were randomly divided into four equal groups (6 rats each) as follows; Group I: (control), Group II: The rats received ACR 45mg/kg/day, Group III: The rats received ACR concomitant with Omega3 at a dosage of 200mg/kg/day, Group IV: The rats received ACR concomitant with Green Tea dissolved in drinking water at a dosage of 5gm/litre. The rats were euthanized 8 weeks from the experiment. Malondialdehyde (MDA) and Glutathione (GSH) were measured in cerebellar homogenates. Sections of 5 µm thickness from specimens from the cerebellum were stained with Hx & E, Silver stain and immunohistochemical stains; PDGFα (for Oligodendrocytes), GFAP (for Astrocytes) and BCL2 (Antiapoptotic).


Omega3 and Green Tea had improved MDA & GSH as compared to ACR group. Histologically, ACR group showed variable degrees of cellular degeneration. Omega3 had induced Oligodendrogenesis in group III. The optical density of silver stain was significantly p<0.05 increased in group III & IV as compared to ACR group. Area % of positive PDGFα was significantly increased in ACR+omega3 group as compared to ACR group. Area % of positive GFAP was significantly decreased in group III & IV as compared to ACR group. Area % of positive BCL2 was significantly increased in omega3 received groups as compared to ACR group.


Concomitant administration of Omega 3 or green Tea with ACR might mitigate its adverse cerebellar changes with an Oligodendrogenic role of Omega 3.


acrylamide; cerebellum; green tea; oligodendrocytes; omega 3; rats

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