Format

Send to

Choose Destination
Mol Cell Proteomics. 2019 Feb;18(2):308-319. doi: 10.1074/mcp.RA118.001124. Epub 2018 Nov 6.

Proteome-wide Analysis of Protein Thermal Stability in the Model Higher Plant Arabidopsis thaliana.

Author information

1
From the ‡Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.
2
§Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, Netherlands.
3
From the ‡Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706; msussman@wisc.edu.

Abstract

Modern tandem MS-based sequencing technologies allow for the parallel measurement of concentration and covalent modifications for proteins within a complex sample. Recently, this capability has been extended to probe a proteome's three-dimensional structure and conformational state by determining the thermal denaturation profile of thousands of proteins simultaneously. Although many animals and their resident microbes exist under a relatively narrow, regulated physiological temperature range, plants take on the often widely ranging temperature of their surroundings, possibly influencing the evolution of protein thermal stability. In this report we present the first in-depth look at the thermal proteome of a plant species, the model organism Arabidopsis thaliana By profiling the melting curves of over 1700 Arabidopsis proteins using six biological replicates, we have observed significant correlation between protein thermostability and several known protein characteristics, including molecular weight and the composition ratio of charged to polar amino acids. We also report on a divergence of the thermostability of the core and regulatory domains of the plant 26S proteasome that may reflect a unique property of the way protein turnover is regulated during temperature stress. Lastly, the highly replicated database of Arabidopsis melting temperatures reported herein provides baseline data on the variability of protein behavior in the assay. Unfolding behavior and experiment-to-experiment variability were observed to be protein-specific traits, and thus this data can serve to inform the design and interpretation of future targeted assays to probe the conformational status of proteins from plants exposed to different chemical, environmental and genetic challenges.

KEYWORDS:

Arabidopsis; Plant Biology; Protein Conformation; Quantification; Tandem Mass Spectrometry; Thermal Proteome Profiling

PMID:
30401684
PMCID:
PMC6356070
[Available on 2020-02-01]
DOI:
10.1074/mcp.RA118.001124
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center