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Br J Nutr. 2019 Jan;121(2):146-154. doi: 10.1017/S0007114518003082. Epub 2018 Nov 7.

Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression of IAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet.

Author information

1
1Faculty of Human Life Sciences,Fuji Women's University,Ishikari 061-3204,Japan.
2
2Institution of Life Sciences and Nutrition,Sapporo 001-0037,Japan.

Abstract

We have recently reported that soluble dietary fibre, glucomannan, increased colonic alkaline phosphatase (ALP) activity and the gene expression without affecting the small-intestinal activity and that colonic ALP was correlated with gut mucins (index of intestinal barrier function). We speculated that dietary fermentable carbohydrates including oligosaccharides commonly elevate colonic ALP and gene expression as well as increase mucin secretion and microbial fermentation. To test this hypothesis, male Sprague-Dawley rats were fed a diet containing 30 % lard with or without 4 % fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), raffinose (RAF) and lactulose (LAC), which are non-digestible oligosaccharides or isomalto-oligosaccharides (IMOS; some digestible oligosaccharides) for 2 weeks. Colon ALP activity, the gene expression and gut luminal variables including mucins, organic acids and microbiota were measured. Colonic ALP was significantly elevated in the FOS, RAF and LAC groups, and a similar trend was observed in the GOS group. Colonic expression of intestinal alkaline phosphatase (IAP -I), an ALP gene, was significantly elevated in the FOS, GOS and RAF groups and tended to be increased in the LAC group. Dietary FOS, GOS, RAF and LAC significantly elevated faecal mucins, caecal n-butyrate and faecal ratio of Bifidobacterium spp. Dietary IMOS had no effect on colonic ALP, mucins, organic acids and microbiota. Colon ALP was correlated with mucins, caecal n-butyrate and faecal Bifidobacterium spp. This study demonstrated that non-digestible and fermentable oligosaccharides commonly elevate colonic ALP activity and the expression of IAP-I, with increasing mucins and microbial fermentation, which might be important for protection of gut epithelial homoeostasis.

KEYWORDS:

Akp3 intestinal alkaline phosphatase 3; Alpl alkaline phosphatase; IAP-I intestinal alkaline phosphatase; Muc2 mucin 2; Muc3 mucin 3; ALP alkaline phosphatase; FOS fructo-oligosaccharides; GOS galacto-oligosaccharides; HF high-fat; IMOS isomalto-oligosaccharides; LAC lactulose; RAF raffinose; TNSALP tissue non-specific alkaline phoshatase; liver/bone/kidney; Alkaline phosphatase; Colonic luminal environment; High-fat diet; Oligosaccharides; Rats

PMID:
30400998
DOI:
10.1017/S0007114518003082

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