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Invest Ophthalmol Vis Sci. 2018 Nov 1;59(13):5312-5319. doi: 10.1167/iovs.18-24914.

An iTRAQ-Based Quantitative Proteomic Analysis of Plasma Proteins in Preterm Newborns With Retinopathy of Prematurity.

Author information

1
Department of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
2
Chair of Pharmacology, Jagiellonian University Medical College, Krakow, Poland.
3
Neonatal and Intensive Care Department, Medical University of Warsaw, Warsaw, Poland.
4
Department of Medical Genetics, Jagiellonian University Medical College, Krakow, Poland.
5
Department of Pediatric Research, Oslo University Hospital, University of Oslo, Oslo, Norway.

Abstract

Purpose:

Retinopathy of prematurity (ROP) is a vision-threatening complication of a premature birth, in which the etiology still remains unclear. Importantly, the molecular processes that govern these effects can be investigated in a perturbed plasma proteome composition. Thus, plasma proteomics may add new insights into a better understanding of the pathogenesis of this disease.

Methods:

The cord and peripheral blood of neonates (≤30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (PMA), respectively. Blood samples were retrospectively subdivided into ROP(+) and ROP(-) groups, according to the development of ROP.

Results:

The quantitative analysis of plasma proteome at both time points revealed 30 protein abundance changes between ROP(+) and ROP(-) groups. After standardization to gestational age, children who developed ROP were characterized by an increased C3 complement component and fibrinogen level at both analyzed time points.

Conclusions:

Higher levels of the complement C3 component and fibrinogen, present in the cord blood and persistent to 36 PMA, may indicate a chronic low-grade systemic inflammation and hypercoagulable state that may play a role in the development of ROP.

PMID:
30398622
DOI:
10.1167/iovs.18-24914

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