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Sci Data. 2018 Nov 6;5:180220. doi: 10.1038/sdata.2018.220.

Comprehensive transcriptome analysis of Sarcophaga peregrina, a forensically important fly species.

Author information

1
Department of Biomedical Sciences, College of Medicine, Korea University, Seoul 02841, Korea.
2
Department of Legal Medicine, College of Medicine, Korea University, Seoul 02841, Korea.
3
Research and Development Center, Insilicogen Inc., Yongin 16954, Gyeonggi-do, Republic of Korea.

Abstract

Sarcophaga peregrina (flesh fly) is a frequently found fly species in Palaearctic, Oriental, and Australasian regions that can be used to estimate minimal postmortem intervals important for forensic investigations. Despite its forensic importance, the genome information of S. peregrina has not been fully described. Therefore, we generated a comprehensive gene expression dataset using RNA sequencing and carried out de novo assembly to characterize the S. peregrina transcriptome. We obtained precise sequence information for RNA transcripts using two different methods. Based on primary sequence information, we identified sets of assembled unigenes and predicted coding sequences. Functional annotation of the aligned unigenes was performed using the UniProt, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. As a result, 26,580,352 and 83,221 raw reads were obtained using the Illumina MiSeq and Pacbio RS II Iso-Seq sequencing applications, respectively. From these reads, 55,730 contigs were successfully annotated. The present study provides the resulting genome information of S. peregrina, which is valuable for forensic applications.

PMID:
30398471
PMCID:
PMC6219405
DOI:
10.1038/sdata.2018.220
[Indexed for MEDLINE]
Free PMC Article

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