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J Agric Food Chem. 2018 Nov 21;66(46):12257-12264. doi: 10.1021/acs.jafc.8b05306. Epub 2018 Nov 13.

Active Components from Sea Buckthorn ( Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis.

Author information

1
Institute of Special Animal and Plant Sciences , Chinese Academy of Agricultural Sciences , Changchun , Jilin 130112 , China.
2
Liaoning Academy of Forestry Science , Shenyang , Liaoning 110032 , China.
3
College of Forestry , Shenyang Agricultural University , Shenyang , Liaoning 110866 , China.

Abstract

Sea buckthorn ( Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammatory factors. Furthermore, these compounds over-regulated the proteins of DNA damage signaling pathway and alpha-smooth muscle actin (α-SMA). Moreover, active components of sea buckthorn berry (ACSB) treatment attenuated fibrosis development in rats after bile duct ligation (BDL), reducing liver injury and inflammation, and reviving liver function in a dose-dependent manner. Moreover, ACSB down-regulated the expression of α-SMA, while over-regulating the DNA damage signaling pathway and the related genes. These suggest that ACSB inhibit DNA repair of HSCs, make them in a damaged state, inhibit the expression of TGF-β, and induce apoptosis.

KEYWORDS:

DNA damage; HSCs; liver fibrosis; sea buckthorn

PMID:
30398061
DOI:
10.1021/acs.jafc.8b05306
[Indexed for MEDLINE]

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