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Anticancer Res. 2018 Nov;38(11):6107-6111. doi: 10.21873/anticanres.12961.

CUB Domain-containing Protein 1 (CDCP1) Is Down-regulated by Active Hexose-correlated Compound in Human Pancreatic Cancer Cells.

Author information

1
Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
2
Research Institute of Cancer Prevention, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
3
Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
4
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Japan.
5
Centre for Molecular Medicine and Biobanking, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
6
Department of Oral and Maxillofacial Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
7
Research Institute of Health Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
8
Research Institute of Cancer Prevention, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan climates@hoku-iryo-u.ac.jp.

Abstract

BACKGROUND/AIM:

We have previously reported that treatment of pancreatic cancer cells with active hexose-correlated compound (AHCC), an extract of a basidiomycete mushroom, decreases the levels of tumor-associated proteins including heat-shock protein 27 (HSP27), heat shock factor 1 (HSF1) and sex-determining region Y-box 2 (SOX2). The transmembrane glycoprotein, CUB domain-containing protein 1 (CDCP1) has been reported to be up-regulated in various cancers, and be associated with invasion and metastasis. The aim of this study was to examine the effect of AHCC on the expression of CDCP1 in KLM1-R cells.

MATERIALS AND METHODS:

Gemcitabine-resistant pancreatic cancer cells (KLM1-R) were treated with AHCC (10 mg/ml) for 48 h. Western blot analysis of cell extracts with anti-CDCP1 or anti-actin antibodies was performed to assess the expression of CDCP1.

RESULTS:

Expression of CDCP1 was reduced by AHCC treatment of KLM1-R cells, whereas expression of actin was not affected. The ratio of intensities of CDCP1/actin in AHCC-treated KLM1-R cells was significantly suppressed (p<0.05) compared to untreated cells.

CONCLUSION:

AHCC down-regulated CDCP1 expression and inhibited the malignant progression of pancreatic cancer cells.

KEYWORDS:

AHCC; CDCP1; metastasis; pancreatic cancer

PMID:
30396925
DOI:
10.21873/anticanres.12961
[Indexed for MEDLINE]

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