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J Gen Virol. 2018 Dec;99(12):1739-1745. doi: 10.1099/jgv.0.001173. Epub 2018 Nov 5.

Detection, infection dynamics and small RNA response against Culex Y virus in mosquito-derived cells.

Author information

1
1​Institute of Infectology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
2
2​Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany.
3
3​German Centre for Infection research, partner side Hamburg-Lübeck-Borstel-Riems, Hamburg, Germany.
4
4​MRC - University of Glasgow Centre of Virus Research, Glasgow, UK.
5
5​Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany.

Abstract

Many insect cell lines are persistently infected with insect-specific viruses (ISV) often unrecognized by the scientific community. Considering recent findings showing the possibility of interference between arbovirus and ISV infections, it is important to pay attention to ISV-infected cell lines. One example is the Entomobirnavirus, Culex Y virus (CYV). Here we describe the detection of CYV using a combination of small RNA sequencing, electron microscopy and PCR in mosquito cell lines Aag2, U4.4 and C7-10. We found CYV-specific small RNAs in all three cell lines. Interestingly, the magnitude of the detected viral RNA genome is variable among cell passages and leads to irregular detection via electron microscopy. Gaining insights into the presence of persistent ISV infection in commonly used mosquito cells and their interactions with the host immune system is beneficial for evaluating the outcome of co-infections with arboviruses of public health concern.

KEYWORDS:

Culex Y virus; RNAi; entomobirnavirus; insect-specific viruses; mosquito cells; persistent infection

PMID:
30394867
DOI:
10.1099/jgv.0.001173

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