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Plant J. 2018 Nov 5. doi: 10.1111/tpj.14145. [Epub ahead of print]

The role of a class III gibberellin 2-oxidase in tomato internode elongation.

Author information

1
Department of Plant Biology, University of California, Davis, One Shields Ave, Davis, CA, 95616, USA.
2
Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), 46022, Valencia, Spain.

Abstract

A network of environmental inputs and internal signaling controls plant growth, development and organ elongation. In particular, the growth-promoting hormone gibberellin (GA) has been shown to play a significant role in organ elongation. The use of tomato as a model organism to study elongation presents an opportunity to study the genetic control of internode-specific elongation in a eudicot species with a sympodial growth habit and substantial internodes that can and do respond to external stimuli. To investigate internode elongation, a mutant with an elongated hypocotyl and internodes but wild-type petioles was identified through a forward genetic screen. In addition to stem-specific elongation, this mutant, named tomato internode elongated -1 (tie-1) is more sensitive to the GA biosynthetic inhibitor paclobutrazol and has altered levels of intermediate and bioactive GAs compared with wild-type plants. The mutation responsible for the internode elongation phenotype was mapped to GA2oxidase 7, a class III GA 2-oxidase in the GA biosynthetic pathway, through a bulked segregant analysis and bioinformatic pipeline, and confirmed by transgenic complementation. Furthermore, bacterially expressed recombinant TIE protein was shown to have bona fide GA 2-oxidase activity. These results define a critical role for this gene in internode elongation and are significant because they further the understanding of the role of GA biosynthetic genes in organ-specific elongation.

KEYWORDS:

Solanum lycopersicum ; bulked segregant analysis; class III GA 2-oxidase; gibberellins; internode elongation; tomato

PMID:
30394600
DOI:
10.1111/tpj.14145

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