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J Vis Exp. 2018 Oct 18;(140). doi: 10.3791/58429.

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue.

Author information

1
CRTD - Center for Regenerative Therapies Dresden, TU Dresden.
2
CRTD - Center for Regenerative Therapies Dresden, TU Dresden; Center for Healthy Aging, TU Dresden; franziska.knopf@tu-dresden.de.

Abstract

Zebrafish are able to regenerate various organs, including appendages (fins) after amputation. This involves the regeneration of bone, which regrows within roughly two weeks after injury. Furthermore, zebrafish are able to heal bone rapidly after trepanation of the skull, and repair fractures that can be easily introduced into zebrafish bony fin rays. These injury assays represent feasible experimental paradigms to test the effect of administered drugs on rapidly forming bone. Here, we describe the use of these 3 injury models and their combined use with systemic glucocorticoid treatment, which exerts bone inhibitory and immunosuppressive effects. We provide a workflow on how to prepare for immunosuppressive treatment in adult zebrafish, illustrate how to perform fin amputation, trepanation of calvarial bones, and fin fractures, and describe how the use of glucocorticoids affects both bone forming osteoblasts and cells of the monocyte/macrophage lineage as part of innate immunity in bone tissue.

PMID:
30394396
PMCID:
PMC6235570
[Available on 2020-10-18]
DOI:
10.3791/58429

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