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Reprod Toxicol. 2018 Dec;82:103-110. doi: 10.1016/j.reprotox.2018.10.008. Epub 2018 Oct 25.

Aqueous extract of Eruca Sativa protects human spermatozoa from mitochondrial failure due to bisphenol A exposure.

Author information

1
Laboratory of Functional Physiology and Valorization of Bioresources-Higher Institute of Biotechnology of Beja, B.P. 382-9000 Beja, University of Jendouba, Tunisia. Electronic address: dhekra.gramik@yahoo.fr.
2
Laboratory of Functional Physiology and Valorization of Bioresources-Higher Institute of Biotechnology of Beja, B.P. 382-9000 Beja, University of Jendouba, Tunisia. Electronic address: rtibikais@yahoo.fr.
3
Laboratory of Functional Physiology and Valorization of Bioresources-Higher Institute of Biotechnology of Beja, B.P. 382-9000 Beja, University of Jendouba, Tunisia. Electronic address: slimen.selmi@gmail.com.
4
Laboratory of Enzymatic Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia. Electronic address: jridimourad@gmail.com.
5
Laboratory of Functional Physiology and Valorization of Bioresources-Higher Institute of Biotechnology of Beja, B.P. 382-9000 Beja, University of Jendouba, Tunisia. Electronic address: sebaihichem@yahoo.fr.
6
Laboratory of Functional Physiology and Valorization of Bioresources-Higher Institute of Biotechnology of Beja, B.P. 382-9000 Beja, University of Jendouba, Tunisia. Electronic address: lamjed.marzouki@ipeis.rnu.tn.
7
Department of Medicine and Unit of Andrology and Reproductive Medicine, University of Padova, Via Giustiniani 2, 35128, Padova, Italy. Electronic address: iva.sabovic@gmail.com.
8
Laboratory of Enzymatic Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia. Electronic address: carlo.foresta@unipd.it.
9
Department of Medicine and Unit of Andrology and Reproductive Medicine, University of Padova, Via Giustiniani 2, 35128, Padova, Italy. Electronic address: detoni.luca@gmail.com.

Abstract

Medicinal plants are suggested to counteract health disorders from chemical pollutants. Here we explored the possible ameliorative effect of Eruca sativa aqueous extract (ESAE) on in vitro acute functional disturbance induced by Bisphenol A (BPA), a disruptor model in human spermatozoa. Phytochemical screening, high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) analysis and 2,2'-azino-bis [3-ethylbenzthiazoline-6-sulphonic acid]/α,α-diphenyl-β-picrylhydrazyl (ABTS/DPPH) tests disclosed antioxidant properties of ESAE, ascribed to polyphenols and flavonoids. The toxicological impact of BPA on sperm viability and motility was detected for concentration greater than 10 μM but co-incubation with ESAE recovered sperm function at low concentration (15.62 μg/ml). BPA reduced mitochondrial membrane potential (ΔΨm), with no impact on plasma membrane potential (ΔΨp). At low doses, ESAE recovered ΔΨm but higher doses were associated with impairment of both ΔΨm and ΔΨp. ESAE protects towards in vitro BPA-mediated toxicity and its possible use as complementary treatment for male reproductive disorders is critically discussed.

KEYWORDS:

Bisphenol A; Eruca sativa aqueous extract; HPLC-ESI-MS analysis; Mitochondria; Sperm function

PMID:
30393182
DOI:
10.1016/j.reprotox.2018.10.008
[Indexed for MEDLINE]

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