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Cell. 2018 Dec 13;175(7):1769-1779.e13. doi: 10.1016/j.cell.2018.09.054. Epub 2018 Nov 1.

Cell Membranes Resist Flow.

Author information

1
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute.
2
Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
3
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.
4
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute. Electronic address: cohen@chemistry.harvard.edu.

Abstract

The fluid-mosaic model posits a liquid-like plasma membrane, which can flow in response to tension gradients. It is widely assumed that membrane flow transmits local changes in membrane tension across the cell in milliseconds, mediating long-range signaling. Here, we show that propagation of membrane tension occurs quickly in cell-attached blebs but is largely suppressed in intact cells. The failure of tension to propagate in cells is explained by a fluid dynamical model that incorporates the flow resistance from cytoskeleton-bound transmembrane proteins. Perturbations to tension propagate diffusively, with a diffusion coefficient Dσ ∼0.024 μm2/s in HeLa cells. In primary endothelial cells, local increases in membrane tension lead only to local activation of mechanosensitive ion channels and to local vesicle fusion. Thus, membrane tension is not a mediator of long-range intracellular signaling, but local variations in tension mediate distinct processes in sub-cellular domains.

KEYWORDS:

cell mechanics; membrane signaling; membrane tension; porous media; rheology

Comment in

PMID:
30392960
PMCID:
PMC6541487
DOI:
10.1016/j.cell.2018.09.054
[Indexed for MEDLINE]
Free PMC Article

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