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Forensic Sci Int Genet. 2019 Jan;38:113-120. doi: 10.1016/j.fsigen.2018.10.008. Epub 2018 Oct 11.

Developmental validation of the DNATyperY26 PCR amplification kit: An enhanced Y-STR multiplex for familial searching.

Author information

1
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: moxiaoting871015@163.com.
2
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: j_zhang@yeah.net.
3
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: 89008801@qq.com.
4
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: 82899503@qq.com.
5
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China.
6
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: zhaoxchun@sina.com.
7
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China; National Engineering Laboratory for Forensic Science, Beijing, 100038, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Beijing 100038, China. Electronic address: j.zhang@live.cn.

Abstract

The DNATyperY26 PCR Amplification kit, which including 26 low-medium mutating Y-STRs, is designed for Y-STR familial searching casework. The kit combines nine new Y-STR loci in addition to the 17 Y-STR loci from the commercially available AmpFlSTR®Yfiler® kit. The validation of the DNATyperY26 kit was performed in terms of technical index, including accuracy, stability, species specificity, sensitivity, adaptability for various samples, and mixture. Further, mutations of the 26 Y-STRs were analyzed by 1167 DNA-confirmed father-son pairs, and the results indicated that these loci had low or medium mutation rates. Furthermore, these Y-STRs loci were also tested in 1072 random male samples from Henan, Shanxi, Inner Mongolia, and Chongqing in China, showing their high power for forensic discrimination in the Chinese population. Thus, the DNATyperY26 PCR Amplification kit is a powerful tool for 'Y-STRs familial searching' in actual sexual-assault cases, indicating its unique advantage in familial searching due to Y-STR loci with only low-medium mutation rates.

KEYWORDS:

DNATyper(™); Familial searching; Forensic science; Y-STR validation

PMID:
30391624
DOI:
10.1016/j.fsigen.2018.10.008
[Indexed for MEDLINE]

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