Format

Send to

Choose Destination
Cryobiology. 2018 Dec;85:1-6. doi: 10.1016/j.cryobiol.2018.10.266. Epub 2018 Nov 2.

Dimethylacetamide and trehalose for ram semen cryopreservation.

Author information

1
School of Veterinary and Animal Science Medicine, Federal University of Bahia, Av. Adhemar de Barros, 500, Salvador, Bahia, 40170-110, Brazil. Electronic address: rfbvet1@gmail.com.
2
College of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, 18618-000, Brazil. Electronic address: euniceoba@fmvz.unesp.br.
3
School of Veterinary and Animal Science Medicine, Federal University of Bahia, Av. Adhemar de Barros, 500, Salvador, Bahia, 40170-110, Brazil.
4
Animal Reproduction Biotechnology Laboratory, Embrapa Coastal Tablelands, Av. Beira Mar 3250, Aracaju, Sergipe, 49025-040, Brazil. Electronic address: hymerson.azevedo@embrapa.br.

Abstract

Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%), and its interaction with trehalose (TRE, 100 mOsmol). A further objective of this study was to evaluate in vivo fertility of ram semen cryopreserved with dimethylacetamide. The control treatment was an extender medium with 6% glycerol (GLY). Five treatments were examined: 6% GLY, 3% DMA, 6% DMA, 3% DMA + TRE, and 6% DMA + TRE. After thawing, the kinetic sperm parameters were analyzed using a computerized system. Sperm viability was observed using a multiple parameter sperm staining with propidium iodide (for plasmatic membrane integrity), JC-1 (for mitochondrial membrane potential), and FITC-PSA (for acrosomal integrity). The isosmotic extender with GLY was the most effective medium for the maintenance of sperm characteristics, compared to extenders containing DMA as cryoprotectant. Regarding fertility, the extender medium with 3% DMA may be a safe alternative for sperm cryopreservation, and does not compromise the fertility rates. The addition of TRE decreased the kinetic sperm parameters; however, a positive effect on the integrity of the plasmatic and acrosomal membranes was observed.

KEYWORDS:

Amide; Frozen semen; Hyperosmotic extender; Ovine

PMID:
30391233
DOI:
10.1016/j.cryobiol.2018.10.266
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center