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Virology. 2019 Jan 2;526:99-104. doi: 10.1016/j.virol.2018.10.011. Epub 2018 Oct 26.

Recombinant fiber-2 protein protects Muscovy ducks against duck adenovirus 3 (DAdV-3).

Author information

1
Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, PR China; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
2
Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, PR China.
3
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
4
Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, PR China. Electronic address: zhqf1012@163.com.

Abstract

As a novel duck adenovirus 3 (DAdV-3) infection caused significant economic losses to the poultry industry in China, there is an urgent need to develop a safe and effective vaccine. In the research, fiber-1 and fiber-2 proteins were expressed and purified, respectively. To evaluate the immunogenicity of the two recombinant proteins, we investigated the IgY antibodies and virus-neutralizing antibodies in duck sera. The protective efficacy was evaluated by mortality, virus shedding and histopathological examinations after challenged with the DAdV-3. Results showed that the IgY antibody levels of the fiber-2 group was significantly higher than that of the fiber-1 group and inactivated vaccine group. Ducks vaccinated with fiber-2 group provided full protection with no mortality, no virus shedding and no histological lesions, superior to other groups. These results suggest that the fiber-2 protein can be an ideal candidate for subunit vaccine against the disease.

KEYWORDS:

DAdV-3; Fiber-1 protein; Fiber-2 protein; Immunogenicity

PMID:
30388631
DOI:
10.1016/j.virol.2018.10.011
[Indexed for MEDLINE]

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