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Gene. 1987;53(2-3):293-8.

Cloning of the riboB locus of Aspergillus nidulans.

Abstract

We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele.

PMID:
3038695
DOI:
10.1016/0378-1119(87)90019-9
[Indexed for MEDLINE]

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