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Cell Biosci. 2018 Oct 26;8:55. doi: 10.1186/s13578-018-0254-y. eCollection 2018.

MicroRNA-29a-3p enhances dental implant osseointegration of hyperlipidemic rats via suppressing dishevelled 2 and frizzled 4.

Liu F#1,2, Wang Z#2,3, Liu F4, Xu J2,3, Liu Q1,2, Yin K5,6, Lan J1,2,7.

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1Department of Prosthodontics, School of Stomatology, Shandong University, Jinan, 250000 China.
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, 44-1 West Wenhua Street, Jinan, 250012 Shandong China.
3Department of Pediatric Dentistry, School of Stomatology, Shandong University, Jinan, 250000 China.
Department of Implantology, Stomatological Hospital of Nanyang, Nanyang, 473000 China.
4Department of Orthodontics, Herman Ostrow School of Dentistry, Los Angeles, CA 90089 USA.
5Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, Los Angeles, 90033 USA.
7Department of Prosthodontics, School of Dentistry, Shandong University, Jinan, China.
Contributed equally



Fine osseointegration is the basis of long-term survival of implant. In our previous study, we observed a strong correlation between hyperlipidemia and compromised osseointegration. MicroRNA-29a-3p (miR-29a-3p) has been discovered to participate in bone marrow mesenchymal stem cells (BMSCs) differentiation. However, the role and the underlying mechanisms of hyperlipidemia and miR-29a-3p in osseointegration still remain obscure.


In peri-implant bone tissues of hyperlipidemia rats, bone mass, mineralization and bone trabecula formation were weakened. Alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2), and miR-29a-3p expression were reduced. While in normal rats, implant-bone interfaces were filled with dense new bone and ALP, Runx2 and miR-29a-3p were up-regulated. Overexpressed miR-29a-3p can reverse the adverse effect of hyperlipidemia on osseointegration. Implants were tightly integrated with the surrounding dense new bone tissues, and ALP as well as Runx2 mRNAs were enhanced in miR-29a-3p overexpressed and hyperlipidemia rats, while little peri-implant bone tissue existed, ALP and Runx2 deregulated on miR-29a-3p inhibited rats. Dishevelled 2 (Dvl2) mRNA was declined in peri-implant bone tissue of high-fat (HF) group than normal group, while frizzled 4 (Fzd4) mRNA declined on day 5 and increased from day 10 to day 20 after implantation in hyperlipidemia rats than in normal rats. Next, BMSCs were cultured under HF or normal medium in vitro. In the HF group, ALP activity and mineralization, ALP and Runx2 mRNAs and proteins expression, and miR-29a-3p expression were suppressed, while adipogenesis was increased, as a result, cytoskeletons were sparse and disordered compared to control group. However, when miR-29a-3p was overexpressed in BMSCs, ALP activity, ALP, Runx2, Dvl2 and Fzd4 mRNAs and proteins expressions were up-regulated. As miR-29a-3p was inhibited in BMSCs, the reverse results were obtained. In addition, promoter assay revealed that miR-29a-3p can directly suppress Wnt/β-catenin pathway related Dvl2 and Fzd4 through binding to their 3'-UTR.


MiR-29a-3p facilitated implant osseointegration via targeting Wnt/β-catenin pathway-related Dvl2 and Fzd4. MiR-29a-3p/Dvl2/Fzd4 may serve as a promising therapeutic target for hyperlipidemia osseointegration.


Dvl2; Fzd4; Hyperlipidemia; Osseointegration; miR-29a-3p

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