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Biol Res. 2018 Nov 1;51(1):42. doi: 10.1186/s40659-018-0193-4.

Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae).

Author information

1
Sociedad de Investigación y Servicios, BioTECNOS Ltda, 49 ½ Oriente, 2385, Parque del Sol, Talca, Chile. biotecnos.rg@gmail.com.
2
Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí, Portoviejo, Manabí, Ecuador. pepinpico@gmail.com.
3
Centro de Biotecnología de los Recursos Naturales, Departamento de Ciencias Forestales, Universidad Católica del Maule, Campus San Miguel, Av. San Miguel 3605, Casilla 617, Talca, Chile. pepinpico@gmail.com.
4
Facultad de Ciencias Agrarias, Universidad de Talca, Talca, Chile. pepinpico@gmail.com.
5
Centro de Biotecnología de los Recursos Naturales, Departamento de Ciencias Forestales, Universidad Católica del Maule, Campus San Miguel, Av. San Miguel 3605, Casilla 617, Talca, Chile. kquiroz@ucm.cl.
6
Facultad de Agronomía, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Macul, Santiago de Chile, Chile.
7
Centro de Biotecnología de los Recursos Naturales, Departamento de Ciencias Forestales, Universidad Católica del Maule, Campus San Miguel, Av. San Miguel 3605, Casilla 617, Talca, Chile.
8
Sociedad de Investigación y Servicios, BioTECNOS Ltda, 49 ½ Oriente, 2385, Parque del Sol, Talca, Chile.
9
Verdant BioScience Pte. Ltda, 18, Duxton Hill, Singapore, 089601, Singapore.

Abstract

BACKGROUND:

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.

RESULTS:

Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively.

CONCLUSIONS:

From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

KEYWORDS:

Chilean berry; Gaultheria pumila; Microsatellite; Pyrosequencing

PMID:
30384861
PMCID:
PMC6211410
DOI:
10.1186/s40659-018-0193-4
[Indexed for MEDLINE]
Free PMC Article

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