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J Agric Food Chem. 2018 Nov 28;66(47):12561-12570. doi: 10.1021/acs.jafc.8b04975. Epub 2018 Nov 14.

Impact of Salicylic Acid Content and Growing Environment on Phytoprostane and Phytofuran (Stress Biomarkers) in Oryza sativa L.

Author information

1
Cátedra de Climatología y Fenología Agrícola, Facultad de Ciencias Agrarias y Forestales , Universidad Nacional de la Plata , Calle 60 y 119 , 1900 La Plata , Buenos Aires , Argentina.
2
Research Group on Quality, Safety and Bioactivity of Plant Foods. Department of Food Science and Technology , Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas , Campus de Espinardo 25 , 30100 Espinardo , Spain.
3
Institut des Biomolécules Max Mousseron, Unité Mixte de Recherche 5247 , University of Montpellier, Centre National de la Recherche Scientifique, and École Nationale Supérieure de Chimie de Montpellier , Montpellier , France.

Abstract

Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are oxylipins synthesized by nonenzymatic peroxidation of α-linolenic acid. These compounds are biomarkers of oxidative degradation in plant foods. In this research, the effect of environment and supplementation with salicylic acid (SA) on PhytoPs and PhytoFs was monitored by ultra-high-performance liquid chromatography coupled to electrospray ionization and triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) on seven rice genotypes from Oryza sativa L. subsp. japonica. The plastic cover environment and spray application with 1 and 15 mM SA produced a reduction in the concentration of most of these newly established stress biomarkers [9-F1t-PhytoP, ent-16-F1t-PhytoP, ent-16- epi-16-F1t-PhytoP, 9-D1t-PhytoP, 9- epi-9-D1t-PhytoP, 16-B1-PhytoP, 9-L1-PhytoP, ent-16( RS)-9- epi-ST-Δ14-10-PhytoF, ent-9( RS)-12- epi-ST-Δ10-13-PhytoF, and ent-16( RS)-13- epi-ST-Δ14-9-PhytoF] by 60.7% on average. The modification observed in the level of PhytoPs and PhytoFs differed according to the specific oxylipins and genotype, demonstrating a close linkage between genetic features and resistance to abiotic stress, to some extent mediated by the sensitivity of plants to the plant hormone SA that participates in the physiological response of higher plants to stress. Thus, in plants exposed to stressing factors, SA contribute to modulating the redox balance, minimizing the oxidation of fatty acids and thus the syntheis of oxylipins. These results indicated that SA could be a promising tool for managing the thermotolerance of rice crop. However, it remains necessary to study the mechanism of action of PhytoPs and PhytoFs in biochemical processes related to the defense of plants and define their role as stress biomarkers through a nonenzymatic pathway.

KEYWORDS:

agronomical practices; air temperature; biomarkers; oxidative stress; rice

PMID:
30384603
DOI:
10.1021/acs.jafc.8b04975
[Indexed for MEDLINE]

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