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BMC Cancer. 2018 Nov 1;18(1):1058. doi: 10.1186/s12885-018-4952-y.

Discovery of a diagnostic biomarker for colon cancer through proteomic profiling of small extracellular vesicles.

Lee CH1,2, Im EJ1,2, Moon PG3,4, Baek MC5,6.

Author information

1
Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea.
2
Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea.
3
Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea. pyonggonmoon@gmail.com.
4
Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea. pyonggonmoon@gmail.com.
5
Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea. mcbaek@knu.ac.kr.
6
Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea. mcbaek@knu.ac.kr.

Abstract

BACKGROUND:

Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information regarding the condition of cells and contribute to the recruitment and reprogramming of components associated with the tumor environment. Therefore, many researchers have suggested that small-EV proteins are potential biomarkers for diseases such as cancer. Colon cancer (CC) is one of the most common causes of cancer-related deaths worldwide. Biomarkers such as carcinoembryonic antigen (CEA) show low sensitivity (~ 40%), and thus the demand for novel biomarkers for CC diagnosis is increasing.

METHODS:

In this study, we identified biomarkers for diagnosing CC through proteomic analysis of small-EVs from CC cell lines. These small-EVs were characterized by western blot analysis, nanoparticle tracking analysis, and transmission electron microscopy and analyzed using mass spectrometry.

RESULTS:

Five selected proteins were found to be upregulated in CC by western blot analysis. Among the candidate proteins, tetraspanin 1 (TSPAN1) was found to be upregulated in plasma EVs from CC patients compared to those from healthy controls (HCs) with 75.7% sensitivity.

CONCLUSIONS:

These results suggest that TSPAN1 is a potent non-invasive biomarker for CC detection. Our experimental strategy provides useful insights into the identification of cancer-specific non-invasive biomarkers.

KEYWORDS:

Biomarker; Colon cancer; Diagnosis; Proteomics; Small extracellular vesicle

PMID:
30382917
PMCID:
PMC6211419
DOI:
10.1186/s12885-018-4952-y
[Indexed for MEDLINE]
Free PMC Article

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