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Nature. 2018 Nov;563(7729):72-78. doi: 10.1038/s41586-018-0654-5. Epub 2018 Oct 31.

Shared and distinct transcriptomic cell types across neocortical areas.

Author information

1
Allen Institute for Brain Science, Seattle, WA, USA. bosiljkat@alleninstitute.org.
2
Allen Institute for Brain Science, Seattle, WA, USA.
3
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
4
Massachusetts Institute of Technology, Cambridge, MA, USA.

Abstract

The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing. Nearly all types of GABA (γ-aminobutyric acid)-containing neurons are shared across both areas, whereas most types of glutamatergic neurons were found in one of the two areas. By combining single-cell RNA sequencing and retrograde labelling, we match transcriptomic types of glutamatergic neurons to their long-range projection specificity. Our study establishes a combined transcriptomic and projectional taxonomy of cortical cell types from functionally distinct areas of the adult mouse cortex.

PMID:
30382198
PMCID:
PMC6456269
DOI:
10.1038/s41586-018-0654-5
[Indexed for MEDLINE]
Free PMC Article

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