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FEBS Lett. 2018 Dec;592(24):4051-4065. doi: 10.1002/1873-3468.13282. Epub 2018 Nov 15.

miR-342-3p promotes osteogenic differentiation via targeting ATF3.

Author information

1
Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, China.
2
Department of Orthopaedics Surgery, Tianjin Medical University General Hospital, Tianjin Medical University, China.
3
School of Medical Imaging, Tianjin Medical University, China.

Abstract

Through their multiple targets, microRNAs (miRNAs) are involved in numerous physiological and pathological processes. In this study, miR-342-3p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR-342-3p impairs osteoblast activity and matrix mineralization, while over expression of miR-342-3p promotes osteoblast differentiation significantly. Moreover, miR-342-3p directly targets activating transcription factor 3 (ATF3), which inhibits transcription of pro-osteogenic differentiation-associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah/Vasp-Like (EVL) locus in undifferentiated pre-osteoblasts; however, demethylation of the EVL CpG island induces over expression of miR-342-3p during osteogenic differentiation. This study suggests that miR-342-3p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.

KEYWORDS:

ATF3; microRNA; ossification; osteogenic differentiation; osteoporosis

PMID:
30381822
DOI:
10.1002/1873-3468.13282
[Indexed for MEDLINE]

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