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J Immunol. 2018 Dec 1;201(11):3465-3470. doi: 10.4049/jimmunol.1800774. Epub 2018 Oct 31.

High-Throughput Single-Cell Sequencing of both TCR-β Alleles.

Author information

1
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109; and.
2
Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109.
3
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109; and engel@umich.edu.

Abstract

Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-β alleles in hundreds of single cells using next-generation sequencing.

PMID:
30381480
DOI:
10.4049/jimmunol.1800774

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