Format

Send to

Choose Destination
Biosensors (Basel). 2018 Oct 30;8(4). pii: E102. doi: 10.3390/bios8040102.

Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters.

Author information

1
Medical Cell BioPhysics Group (MCBP), University of Twente, 7500 AE Enschede, The Netherlands. r.b.m.schasfoort@utwente.nl.
2
Interfluidics BV, 7483 AL Haaksbergen, The Netherlands. r.b.m.schasfoort@utwente.nl.
3
Medical Cell BioPhysics Group (MCBP), University of Twente, 7500 AE Enschede, The Netherlands. f.abali@utwente.nl.
4
Medical Cell BioPhysics Group (MCBP), University of Twente, 7500 AE Enschede, The Netherlands. ivanstojanovic172@msn.com.
5
Interfluidics BV, 7483 AL Haaksbergen, The Netherlands. ivanstojanovic172@msn.com.
6
Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, 1066 CX Amsterdam, The Netherlands. G.Vidarsson@sanquin.nl.
7
Medical Cell BioPhysics Group (MCBP), University of Twente, 7500 AE Enschede, The Netherlands. l.w.m.m.terstappen@utwente.nl.

Abstract

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

KEYWORDS:

SPR imaging; cytometry; single-cell secretion; surface plasmon resonance

PMID:
30380705
DOI:
10.3390/bios8040102
Free full text

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI)
Loading ...
Support Center