Send to

Choose Destination
PLoS One. 2018 Oct 31;13(10):e0206667. doi: 10.1371/journal.pone.0206667. eCollection 2018.

Determination of the presence of 5-methylcytosine in Paramecium tetraurelia.

Author information

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, Bern, Switzerland.
Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, Bern, Switzerland.
Tocagen Incorporated, San Diego, California, United States of America.
Storm Therapeutics Limited, Moneta Building, Babraham Research Campus, Cambridge, United Kingdom.


5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methylcytosine DNA methylation as an integral part of its epigenetic arsenal.

[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

Daniel J. Hogan is employed by Tocagen Incorporated. Alan G. Hendrick is employed by Storm Therapeutics Limited. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center