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Methods Mol Biol. 2019;1882:197-206. doi: 10.1007/978-1-4939-8879-2_18.

Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells.

Author information

1
Laboratory of Molecular Pathology and Nanobioimaging, Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy.
2
Laboratory of Molecular Pathology and Nanobioimaging, Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy. ciro.isidoro@med.uniupo.it.

Abstract

Macroautophagy is a catabolic process through which redundant, aged, or damaged cellular structures are first enclosed within double-membrane vesicles (called autophagosomes), and thereafter degraded within lysosomes. Macroautophagy provides a primary route for the turnover of macromolecules, membranes and organelles, and as such plays a major role in cell homeostasis. As part of the stress response, autophagy is crucial to determine the cell fate in response to extracellular or intracellular injuries. Autophagy is involved in cancerogenesis and in cancer progression. Here we illustrate the essential methods for monitoring autophagy in pancreatic cancer cells.

KEYWORDS:

Autophagosome; Autophagy; LC3; Lysosome; Pancreatic cancer; p62

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