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Methods Mol Biol. 2019;1882:55-62. doi: 10.1007/978-1-4939-8879-2_5.

Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia.

Author information

1
Department of Cell Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX, USA.
2
Department of Cell Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX, USA. wangp3@uthscsa.edu.

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

KEYWORDS:

3D culture; Acinar-to-ductal metaplasia; Flow cytometry; Human exocrine pancreatic cells; Lineage tracing

PMID:
30378043
DOI:
10.1007/978-1-4939-8879-2_5
[Indexed for MEDLINE]

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