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Nat Methods. 2018 Nov;15(11):905-908. doi: 10.1038/s41592-018-0173-1. Epub 2018 Oct 30.

A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps.

Author information

1
Los Alamos National Laboratory, Los Alamos, NM, USA. tterwilliger@newmexicoconsortium.org.
2
New Mexico Consortium, Los Alamos, NM, USA. tterwilliger@newmexicoconsortium.org.
3
Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
4
Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
5
Department of Physics and International Centre for Quantum and Molecular Structures, Shanghai University, Shanghai, China.

Abstract

We report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix.map_to_model. We applied our approach to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at resolutions worse than 3 Å.

PMID:
30377346
PMCID:
PMC6214191
DOI:
10.1038/s41592-018-0173-1
[Indexed for MEDLINE]
Free PMC Article

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