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Environ Sci Technol. 2018 Nov 20;52(22):13501-13510. doi: 10.1021/acs.est.8b04169. Epub 2018 Nov 8.

Changes in microRNA-mRNA Signatures Agree with Morphological, Physiological, and Behavioral Changes in Larval Mahi-Mahi Treated with Deepwater Horizon Oil.

Author information

1
Department of Environmental Sciences , University of California , Riverside , California 92521 , United States.
2
Department of Biological Sciences & Advanced Environmental Research Institute , University of North Texas in Denton , Denton , Texas 76203 , United States.
3
Department of Marine Biology and Ecology, RSMAS , University of Miami , Miami , Florida 33149 , United States.

Abstract

In this study, we performed a systematic evaluation of global microRNA-mRNA interactions associated with the developmental toxicity of Deepwater Horizon oil using a combination of integrated mRNA and microRNA deep sequencing, expression profiling, gene ontology enrichment, and functional predictions by a series of advanced bioinformatic tools. After exposure to water accommodated fraction (WAF) of both weathered slick oil (0.5%, 1%, and 2%) and source oil (0.125%, 0.25%, and 0.5%) from the Deep Water Horizon oil spill, four dose-dependent miRNAs were identified, including three up-regulated (miR-23b, miR-34b, and miR-181b) and one down-regulated miRNAs (miR-203a) in mahi-mahi hatchings exposed from 6 h postfertilization (hpf) to 48 hpf. Consistent with morphological, physiological, and behavioral changes, the target genes of these miRNAs were largely involved in the development of the cardiovascular, visual, nervous system and associated toxicity pathways, suggesting that miRNAs play an essential role in regulating the responses to oil exposure. The results obtained from this study improve our understanding of the role of miRNAs and their target genes in relation to dose-dependent oil toxicity and provide the potential of using miRNAs as novel biomarkers in future oil studies.

PMID:
30376307
DOI:
10.1021/acs.est.8b04169

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