Format

Send to

Choose Destination
J Cereb Blood Flow Metab. 2020 Jan;40(1):204-213. doi: 10.1177/0271678X18809886. Epub 2018 Oct 30.

Divergent metabolic substrate utilization in brain during epileptogenesis precedes chronic hypometabolism.

Author information

1
Department of Nuclear Medicine, Hannover Medical School, Hannover, Germany.
2
Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine, Hannover, Germany.

Abstract

Alterations in metabolism during epileptogenesis may be a therapy target. Recently, an increase in amino acid transport into the brain was proposed to play a role in epileptogenesis. We aimed to characterize alterations of substrate utilization during epileptogenesis and in chronic epilepsy. The lithium-pilocarpine post status epilepticus (SE) rat model was used. We performed longitudinal O-(2-[(18)F]fluoroethyl)-l-tyrosine (18F-FET) and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and calculated 18F-FET volume of distribution (Vt) and 18F-FDG uptake. Correlation analyses were performed with translocator protein-PET defined neuroinflammation from previously acquired data. We found reduced 18F-FET Vt at 48 h after SE (amygdala: -30.2%, p = 0.014), whereas 18F-FDG showed increased glucose uptake 4 and 24 h after SE (hippocampus: + 43.6% and +42.5%, respectively; p < 0.001) returning to baseline levels thereafter. In chronic epileptic animals, we found a reduction in 18F-FET and 18F-FDG in the hippocampus. No correlation was found for 18F-FET or 18F-FDG to microglial activation at seven days post SE. Whereas metabolic alterations do not reflect higher metabolism associated to activated microglia, they might be partially driven by chronic neuronal loss. However, both metabolisms diverge during early epileptogenesis, pointing to amino acid turnover as a possible biomarker and/or therapeutic target for epileptogenesis.

KEYWORDS:

Amino acid turnover; epilepsy; fluorodeoxyglucose; fluoroethyl-l-tyrosine; positron emission tomography

PMID:
30375913
PMCID:
PMC6928550
[Available on 2021-01-01]
DOI:
10.1177/0271678X18809886

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center