Format

Send to

Choose Destination
Thyroid. 2018 Oct 30. doi: 10.1089/thy.2018.0068. [Epub ahead of print]

Regeneration of a bioengineered thyroid using decellularized thyroid matrix.

Author information

1
Thyroid Disease Diagnosis and Treatment Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China ; panjun1928@aliyun.com.
2
Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China ; felixcat111@163.com.
3
Hangzhou, China ; fangyun_butterfly@163.com.
4
Hangzhou, China ; ander_syb@aliyun.com.
5
Hangzhou, China ; zhou.xue.yu@163.com.
6
Hangzhou, China ; Lwsjn@zju.edu.cn.
7
Hangzhou, China ; 10918188@zju.edu.cn.
8
Hangzhou, China ; 670354994@qq.com.
9
Hangzhou, China ; 415151785@qq.com.
10
Hangzhou, China ; wangyan07@zju.edu.cn.
11
Hangzhou, China ; zxh828@hotmail.com.
12
Hangzhou, China ; cynthia.w@163.com.
13
Thyroid Disease Diagnosis and Treatment Center, The First Affiliated Hospital, College of Medicine, Zhejiang University , 79# Qingchun Road, Hangzhou 310003, People's Republic China , Hangzhou, Zhejiang, China , 310000.
14
United States ; wuwu5925@zju.edu.cn.

Abstract

BACKGROUND:

Hypothyrodism is a common hormone deficiency condition. Regenerative medicine approaches, such as a bioengineered thyroid, have been proposed as potential therapeutic alternatives for patients with hypothyroidism. Here we demonstrate a novel approach to generate thyroid grafts using decellularized rat thyroid matrix.

MATERIALS AND METHODS:

Isolated rat thyroid glands were perfused with 1% SDS to generate a decellularized thyroid scaffold. The rat thyroid scaffold was then recellularized with rat thyroid cell line to reconstruct the thyroid by perfusion-seeding technique. As a pilot study, the decellularized rat thyroid scaffold was perfused with human-derived thyrocytes and parathyroid cells.

RESULTS:

The decellularization process retained the intricate three-dimensional microarchitecture with a perfusable vascular network and native extracellular matrix (ECM) components, allowing efficient reseeding of the thyroid matrix with the FRTL-5 rat thyroid cell line generating three-dimensional follicular structures in vitro. In addition, the recellularized thyroid showed successful cellular engraftment and thyroid-specific function including synthesis of thyroglobulin and thyroid peroxidase. Moreover, the decellularized rat thyroid scaffold could further be recellularized with human-derived thyroid cells and parathyroid cells to reconstruct a humanized bioartificial endocrine organ, which maintained expression of critical genes such as thyroglobulin, thyroid peroxidase and parathyroid hormone.

CONCLUSION:

These findings demonstrate the utility of a decellularized thyroid ECM scaffold system for the development of functional, bioengineered thyroid tissue, which could potentially be used to treat hypothyroidism.

PMID:
30375266
DOI:
10.1089/thy.2018.0068

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center