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Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):11796-11801. doi: 10.1073/pnas.1813143115. Epub 2018 Oct 29.

Engineering yeast endosymbionts as a step toward the evolution of mitochondria.

Author information

1
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037.
2
Department of Anatomy, School of Medicine, University of California, San Francisco, CA 94158.
3
Core Microscopy Facility, The Scripps Research Institute, La Jolla, CA 92037.
4
Department of Advanced Imaging and Microscopy, Oak Crest Institute of Science, Monrovia, CA 91016.
5
Department of General Medical Biology, Genomics Institute of the Novartis Research Foundation, La Jolla, CA 92121 fsupek@gnf.org schultz@scripps.edu.
6
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037; fsupek@gnf.org schultz@scripps.edu.

Abstract

It has been hypothesized that mitochondria evolved from a bacterial ancestor that initially became established in an archaeal host cell as an endosymbiont. Here we model this first stage of mitochondrial evolution by engineering endosymbiosis between Escherichia coli and Saccharomyces cerevisiae An ADP/ATP translocase-expressing E. coli provided ATP to a respiration-deficient cox2 yeast mutant and enabled growth of a yeast-E. coli chimera on a nonfermentable carbon source. In a reciprocal fashion, yeast provided thiamin to an endosymbiotic E. coli thiamin auxotroph. Expression of several SNARE-like proteins in E. coli was also required, likely to block lysosomal degradation of intracellular bacteria. This chimeric system was stable for more than 40 doublings, and GFP-expressing E. coli endosymbionts could be observed in the yeast by fluorescence microscopy and X-ray tomography. This readily manipulated system should allow experimental delineation of host-endosymbiont adaptations that occurred during evolution of the current, highly reduced mitochondrial genome.

KEYWORDS:

ADP/ATP translocase; endosymbiotic theory; evolution; mitochondria

PMID:
30373839
PMCID:
PMC6243291
[Available on 2019-05-13]
DOI:
10.1073/pnas.1813143115

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