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Eur J Dent. 2018 Oct-Dec;12(4):585-589. doi: 10.4103/ejd.ejd_422_18.

Cytotoxicity evaluation of a new ozonized olive oil.

Author information

Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Section of Dentistry, University of Pavia, Pavia, Italy.
Departement of Molecular Medicine, Unit of Biochemistry, University of Pavia, Pavia, Italy.



The cytocompatibility of a new ozonized olive oil toward immortalized human gingival fibroblasts (HGFs) was evaluated and compared with two common antimicrobial agents based on chlorhexidine digluconate (CHX).

Materials and Methods:

The cytocompatibility of the samples was tested on immortalized HGF-1 cells by 3-(4, 5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were incubated for 2 or 24 h with increasing dilution of ozonized olive oil or CHX agents. The percentage of viable cells was calculated relative to control cells set to 100%.


The ozonized olive oil is cytocompatible, and the viability values of the cells treated for 2 or 24 h with increasing concentrations of ozonized olive oil were significantly higher (P < 0.01) compared with the values obtained using CHX.


The present data demonstrate that due to its cytocompatibility, the new ozonized olive oil could be considered an alternative antibacterial agent.


3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide test; cytotoxicity; gingival fibroblast; ozonized olive oil

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