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Methods Mol Biol. 2019;1889:319-330. doi: 10.1007/978-1-4939-8897-6_19.

Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies.

Author information

1
Nofima AS, Ås, Norway. Tone-Kari.Ostbye@Nofima.no.
2
Nofima AS, Ås, Norway.

Abstract

This chapter outlines methods for isolating myosatellites from Atlantic salmon (Salmo salar), how to keep them in culture and differentiate them into mature myocytes. The protocol further describes how to trans-differentiate the myocytes into osteoblasts (bone cells).

KEYWORDS:

Atlantic salmon; Differentiation; In vitro; Muscle cell; Myosatellite; Trans-differentiation

PMID:
30367423
DOI:
10.1007/978-1-4939-8897-6_19
[Indexed for MEDLINE]

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