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Int J Mol Sci. 2018 Oct 25;19(11). pii: E3327. doi: 10.3390/ijms19113327.

Protective Effects of Euphrasia officinalis Extract against Ultraviolet B-Induced Photoaging in Normal Human Dermal Fibroblasts.

Author information

1
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. y.liu0915@khu.ac.kr.
2
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. firsthes@khu.ac.kr.
3
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. hienngo211@khu.ac.kr.
4
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. harijai2004@gmail.com.
5
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. yeonjukim@khu.ac.kr.
6
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. lilu@khu.ac.kr.
7
College of Life Sciences, Kyung Hee University, 1732, Deogyeong daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea. drhoo@khu.ac.kr.

Abstract

Ultraviolet (UV) radiation induces skin photoaging, which is associated with the elevation of matrix metalloproteinases (MMPs) and the impairment of collagen. The Euphrasia species play a well-known role in the treatment of certain eye disorders through their anti-oxidative and anti-inflammatory activities. However, their protective activity toward UVB-induced damage remains unclear. In the present study, we investigated the protective effect of Euphrasia officinalis (95% ethanol extract) on UVB-irradiated photoaging in normal human dermal fibroblasts (NHDFs). Our results show that Euphrasia officinalis extract exhibited obvious reactive oxygen species (ROS) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, enhanced NHDF cell migration, and reduced UVB-induced apoptosis. The UVB-induced increases in MMP-1 and MMP-3 and decrease in type I procollagen were ameliorated by Euphrasia officinalis treatment, which worked by suppressing the mitogen-activated protein kinase (MAPK) and nuclear transcription factor activator protein 1 (AP-1) signaling pathways. Taken together, our data strongly suggest that Euphrasia officinalis ethanol extract could reduce UVB-induced photoaging by alleviating oxidative stress, proinflammatory activity, and cell apoptosis.

KEYWORDS:

Euphrasia officinalis; apoptosis; matrix metalloproteinases; photoaging; type I procollagen; ultraviolet B

PMID:
30366440
PMCID:
PMC6275060
DOI:
10.3390/ijms19113327
[Indexed for MEDLINE]
Free PMC Article

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