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Gene. 2019 Jun 20;702:194-204. doi: 10.1016/j.gene.2018.10.049. Epub 2018 Oct 23.

The influences of red wine in phenotypes of human cancer cells.

Author information

1
Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, China.
2
Affiliated Cancer Hospital & Institute of Guangzhou Medical University, China; Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
3
Department of Pharmacology, Shantou University Medical College, China; Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
4
Affiliated Cancer Hospital & Institute of Guangzhou Medical University, China.
5
Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. Electronic address: szhong@usc.edu.

Abstract

Alcohol intake increases the risk of cancer development. Approximately 3.6% human cancers worldwide derive from chronic alcohol drinking, including oral, liver, breast and other organs. Our studies in vivo and in vitro have demonstrated that diluted ethanol increase RNA Pol III gene transcription and promotes cell proliferation and transformation, as well as tumor formation. However, it is unclear about the effect of red wines on the human cancer cells. In present study, we investigated the roles of red wine in human cancer cell growth, colony formation and RNA Pol III gene transcription. Low concentration (12.5 mM to 25 mM) of ethanol enhances cell proliferation of breast and esophageal cancer lines, whereas its higher concentration (100 mM to 200 mM) slightly decreases the rates. In contrast, red wines significantly repress cell proliferation of different human cancer lines from low dose to high dose. The results reveal that the red wine also inhibits colony formation of human breast cancer and esophageal carcinoma cells. The effects of repression on different human cancer lines are in a dose-dependent manner. Further analysis indicates that ethanol increases RNA Pol III gene transcription, whereas the red wines significantly reduce transcription of the genes. Interestingly, the effects of mature wine (brick red) on cancer cell phenotypes are much stronger than young wine (intense violet). Together, these new findings suggest that red wines may contain some bioactive components, which are able to inhibit human cancer cell growth and colony formation.

KEYWORDS:

Cell growth; Colony formation; Ethanol; Human cancer; Pol III genes; Red wine

PMID:
30366081
PMCID:
PMC6478559
[Available on 2020-06-20]
DOI:
10.1016/j.gene.2018.10.049
[Indexed for MEDLINE]

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