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Lab Chip. 2018 Dec 7;18(23):3598-3605. doi: 10.1039/c8lc00514a. Epub 2018 Oct 26.

Microfluidic bead encapsulation above 20 kHz with triggered drop formation.

Author information

1
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA.
2
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences (QB3), Chan Zuckerberg Biohub, University of California, San Francisco, San Francisco, CA, USA. adam@abatelab.org.

Abstract

Microsphere beads are functionalized with oligonucleotides, antibodies, and other moieties to enable specific detection of analytes. Droplet microfluidics leverages this for single-molecule or -cell analysis by pairing beads and targets in water-in-oil droplets. Pairing is achieved with devices operating in the dripping regime, limiting throughput. Here, we describe a pairing method that uses beads to trigger the breakup of a jet into monodispersed droplets. We use the method to pair 105 Human T cells with polyacrylamide beads ten times faster than methods operating in the dripping regime. Our method improves the throughput of bead-based droplet workflows, enabling analysis of large populations and the detection of rare events.

PMID:
30362490
PMCID:
PMC6251341
DOI:
10.1039/c8lc00514a
[Indexed for MEDLINE]
Free PMC Article

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