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J Cell Biochem. 2019 Apr;120(4):6330-6338. doi: 10.1002/jcb.27920. Epub 2018 Oct 25.

Long noncoding RNA XIST participates in bladder cancer by downregulating p53 via binding to TET1.

Author information

1
Department of Urology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
2
Department of Urology, Huai'an Second People's Hospital and The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.
3
Department of Urology, Huai'an People's Hospital of Hongze District, Huai'an, China.

Abstract

Long noncoding RNAs (lncRNAs) have been reported to take part in intracellular RNA regulatory networks and play important roles in a lot of pathological processes. Currently, lncRNA X-inactive specific transcript (XIST) has been proved to regulate cell migration, proliferation, and apoptosis in a series of cancers. In this study, we explored whether XIST can affect cell proliferation, migration, and apoptosis in bladder cancer. The influences of XIST on the cell proliferation, cell cycle, and apoptosis were detected in bladder cancer cells. The effect of XIST on the migration of bladder cancer cells was examined by cell migration assay. Through RNA immunoprecipitation and chromatin immunoprecipitation assays, the binding relationship between XIST and TET1 and the regulatory mechanism of TET1 on p53 were examined. Western blot was performed to detect p53 after knockdown or overexpression of XIST. We found that knockdown of XIST suppressed cell migration and proliferation in vitro. Mechanistically, we confirmed that lncRNA XIST participates in bladder cancer by downregulating p53 via binding to TET1. In conclusion, our research revealed the potential role of XIST-TET1-p53 regulatory network in bladder cancer.

KEYWORDS:

10 to 11 translocation 1; X-inactive specific transcript; bladder cancer; long noncoding RNA; p53

PMID:
30362292
DOI:
10.1002/jcb.27920

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