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Folia Microbiol (Praha). 2019 May;64(3):361-372. doi: 10.1007/s12223-018-0659-3. Epub 2018 Oct 25.

Secretomic analysis of Beauveria bassiana related to cattle tick, Rhipicephalus microplus, infection.

Author information

1
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av Ipiranga, 2752, Porto Alegre, RS, 90610-000, Brazil.
2
Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, R. Ramiro Barcelos, 2350, Porto Alegre, RS, 90035-903, Brazil.
3
Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Rod BR 465, km 7, Seropédica, RJ, 23890-000, Brazil.
4
Univates, Av Avelino Talini, 171, Lajeado, RS, 95914-014, Brazil.
5
Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Av Bento Gonçalves, 9500, Porto Alegre, RS, 91501-970, Brazil.
6
Department of Chemical Physiology and Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA, 92037, USA.
7
Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Tv. Primeira Brejinhos, 540-736, Cruz das Almas, BA, 44380-000, Brazil.
8
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av Ipiranga, 2752, Porto Alegre, RS, 90610-000, Brazil. walter.beys@ufrgs.br.
9
Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, R. Ramiro Barcelos, 2350, Porto Alegre, RS, 90035-903, Brazil. walter.beys@ufrgs.br.

Abstract

Beauveria bassiana is widely studied as an alternative to chemical acaricides in controlling the cattle tick Rhipicephalus microplus. Although its biocontrol efficiency has been proved in laboratory and field scales, there is a need to a better understanding of host interaction process at molecular level related to biocontrol activity. In this work, applying a proteomic technique multidimensional protein identification technology (MudPIT), the differential secretome of B. bassiana induced by the host R. microplus cuticle was evaluated. The use of the host cuticle in a culture medium, mimicking an infection condition, is an established experimental model that triggers the secretion of inducible enzymes. From a total of 236 proteins, 50 proteins were identified exclusively in infection condition, assigned to different aspects of infection like host adhesion, cuticle penetration and fungal defense, and stress. Other 32 proteins were considered up- or down-regulated. In order to get a meaningful global view of the secretome, several bioinformatic analyses were performed. Regarding molecular function classification, the highest number of proteins in the differential secretome was assigned in to hydrolase activity, enzyme class of all cuticle-degrading enzymes like lipases and proteases. These activities were also further validated through enzymatic assays. The results presented here reveal dozens of specific proteins and different processes potentially implicated in cattle tick infection improving the understanding of molecular basis of biocontrol of B. bassiana against R. microplus.

PMID:
30361880
DOI:
10.1007/s12223-018-0659-3

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