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Science. 2018 Dec 7;362(6419):1186-1189. doi: 10.1126/science.aat7925. Epub 2018 Oct 25.

Viscous control of cellular respiration by membrane lipid composition.

Budin I1,2, de Rond T3,4, Chen Y3,5, Chan LJG3, Petzold CJ3,5, Keasling JD1,2,6,7,8,9.

Author information

1
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA. keasling@berkeley.edu budin@berkeley.edu.
2
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
3
Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA.
4
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
5
Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
6
Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
7
QB3 Institute, University of California, Berkeley, Berkeley, CA 94270, USA.
8
The Novo Nordisk Foundation Center for Sustainability, Technical University of Denmark, Denmark.
9
Center for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes for Advanced Technologies, Shenzhen, China.

Abstract

Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coli allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.

Comment in

PMID:
30361388
DOI:
10.1126/science.aat7925
[Indexed for MEDLINE]

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