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J Chromatogr A. 2018 Dec 7;1579:129-137. doi: 10.1016/j.chroma.2018.10.018. Epub 2018 Oct 15.

Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry.

Author information

1
Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, Martí i Franquès 1-11, 3rd floor, 08028, Barcelona, Spain. Electronic address: fbenavente@ub.edu.
2
Department of Chemical Engineering and Analytical Chemistry, Institute for Research on Nutrition and Food Safety (INSA·UB), University of Barcelona, Martí i Franquès 1-11, 3rd floor, 08028, Barcelona, Spain.
3
Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.

Abstract

We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.

KEYWORDS:

Bioactive peptides; Capillary electrophoresis; Chemometrics; Foodomics; Mass spectrometry; Peptidomics

PMID:
30361036
DOI:
10.1016/j.chroma.2018.10.018
[Indexed for MEDLINE]

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